rod and/or Mini-BeadBeater (Biospec Products, Inc., Bartlesville, Okla.). Total genomic DNA was extracted with the DNeasy Plant Mini Kit (Qiagen, Santa Clarita, Calif.). Polymerase chain reactions (PCR) were done using specific primers for each of the plastid genes (see Yoon, Hackett, and Bhattacharya 2002; Yoon et al. 2002). Four (A) Mini-BeadBeater (BioSpec), a typical benchtop instrument. (B) OmniLyse bead blender (Claremont BioSolutions), a miniaturized, disposable, battery-operated device. Article Snippet: The Mini-BeadBeater and the Mini-BeadBeater-8 were purchased from BioSpec (Bartlesville, OK). Techniques: The Mini-BeadBeater-16: disrupts microbial cells and plant and animal tissue by violently agitating four to sixteen 2 ml screw-cap tubes containing small ceramic or steel beads and disruption buffer. completely homogenises resistant samples like yeast, spores or fibrous tissue are completely homogenized in around 3 minutes in 0.1 to 1 ml of ...

Mini beadbeater plus

Lesson 4 interior and exterior angles of circles answersusing human embryonic kidney (HEK)-Blue-mTLR5 and HEK-Blue-mTLR4 cells, respectively (Invivogen, Toulouse, France). We resuspended fecal material in PBS to a final concentration of 100 mg/mL and homogenized for 10 seconds using a Mini-Beadbeater-24 (BioSpec, Bartlesville, OK) without the addition of beads to avoid bacteria disruption. The mixture was shaken for 1 min in a mini-Beadbeater (Biospecifics Technologies Corp.) on “fast” speed. For seed tocopherol determination, 10 mg of mature seed was added to 1 g of microbeads (Biospecifics Technologies Corp.) in a sterile microfuge tube to which 500 μL of 1% (w/v) pyrogallol (Sigma) in ethanol was added. Adjectives to describe faithThe Mini-Beadbeater-16 is a high-energy cell disrupter designed to handle four to sixteen samples at a time. The Mini-BeadBeater-16 disrupts microbial cells and plant and animal tissue by violently agitating four to sixteen 2 ml screw-cap microvials containing small glass, ceramic or steel beads and disruption buffer. Please note, the Mini-Beadbeater-96 is compatible with 96 deep well plates. When using deep well microplates possible leakage and subsequent cross contamination between wells is a concern. While the entire mat surface area is evenly clamped to the microplate during beadbeating in the Mini-BeadBeater-96, the user must first thoroughly press the ... The Mini-Beadbeater-24 disrupts up to 24 microbial or tissue samples with better than 95 percent efficiency. Cells are disrupted quickly and safely in the sealed system. The apparatus is easy to clean, has a small footprint and is essentially maintenance free. Compared to the Minibeadbeater-16, this Beadbeater adds advanced electronics for enhanced motor function, continuous variable shaking… Oct 15, 2003 · Hc RNA was prepared with TRIzol Reagent (Life Technologies Inc.) from yeast cells disrupted with a Mini-BeadBeater-8 (BioSpec Products Inc., Bartlesville, Oklahoma, USA). for 5 min in a Mini-BeadBeater-8 (Biospec Products), then cen-trifuged at ∼13,000 g for 10 min. The level of hyaluronic acid in aqueous phase was determined using a hyaluronic acid test kit (Corgenix) according to the manufacturer’s instructions. Fibronectin-binding assays. Fibronectin was bound to 96-well plates (Costar) as described ... Oct 13, 2019 · This mix was autoclaved at 134 °C for 1 h. One mL of the autoclaved sample was smashed using a mini-beadbeater (BioSpec Products Inc., USA) to fully release the starch from the cells, and 0.5 mL of the smashed sample was mixed with 0.5 mL of the sodium phosphate buffer then subjected to the double digestions aforementioned. Oct 15, 2003 · Hc RNA was prepared with TRIzol Reagent (Life Technologies Inc.) from yeast cells disrupted with a Mini-BeadBeater-8 (BioSpec Products Inc., Bartlesville, Oklahoma, USA). Jun 23, 2020 · A total of 2 μg of total RNA (RNeasy Mini Plus, QIAGEN) was used to reverse transcribe cDNA (High-Capacity cDNA Reverse Transcription, Thermo Fisher Scientific). qPCR was then performed on the cDNA (StepOnePlus System, Thermo Fisher Scientific) using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific) and probes (all from Thermo Fisher ... pieces were crushed and powdered by using mini-beadbeater for four mins. Afterward, the sample was put into a waterbath of 65oC for 60 mins, in which the microtube was turned upside down in every 10 mins. The sample was then taken from the waterbath and allowed to cool down at room temperature for two mins, after which, 500 µl Nov 25, 2020 · After addition of buffer C1, samples were incubated at 65°C for 10 min and then subjected to homogenization using the Biospec Mini-Beadbeater (BioSpec Products) for 2 min. An additional wash step with 100% ethanol was included preceding the wash with kit buffer C5. Analytical Abstracts is a current awareness and information retrieval service for analytical chemistry, published by the Royal Society of Chemistry in Cambridge, United Kingdom. It was first published in the mid-1950s by the Society for Analytical What is claimed is: 1. An isolated polynucleotide comprising: (a) a nucleotide sequence encoding a polypeptide having delta-9 elongase activity, wherein the polypeptide has at least 70% amino acid identity, based on the Clustal V method of alignment, when compared to an amino acid sequence as set forth in SEQ ID NO:5 or SEQ ID NO:127; (b) a nucleotide sequence encoding a polypeptide having ... (A and B) Serial dilutions of the targeted microorganism (TMO) plus a fixed amount of internal control (IC) genomic DNA were processed in triplicate using an OmniLyse device for 1.5 min or a BioSpec Mini-BeadBeater/Mini-BeadBeater-8 Article Snippet: The Mini-BeadBeater and the Mini-BeadBeater-8 were purchased from BioSpec (Bartlesville, OK). The new Mini-BeadBeater-Plus™ is a high-energy bead-mill designed to violently agitate up to a half gram sample of biological material sealed inside a 2 ml microcentrifuge vial containing tiny glass, zirconia or steel lysing beads. The Mini-Beadbeater-Plus™ is an upgraded version of the venerable Mini-BeadBeater-1. Improvements include a programmable timer, a powerful brushless motor and 115/230 VAC compatibility. The high-energy bead-mill rapidly and completely disrupts microorganisms and plant and animal tissue. lysed in a Mini-BeadBeater-1™ (BioSpec Products, Inc.). The pF1K T7 Flexi® Vector allows expres-sion of native (untagged) proteins using the T7 RNA polymerase promoter.The vector contains the kanamycin-resistance gene for selection in E.coli. PROTEIN EXPRESSION NUMBER 94 AUGUST 2006 WWW.PROMEGA.COM 28 KRX Genotype Aug 09, 2011 · Samples were mechanically disrupted in a Mini-beadbeater-8TM cell disrupter (BioSpec Products, Inc., Bartlesville, OK) for 10 min. After disruption, samples were incubated on ice for 5 min and the beads were allowed to settle out of the lysis mixture. Samples underwent centrifugation (in an Eppendorf centrifuge at 2100 rcf 1 min).